| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020708 |
| Chromosome: | chromosome 10 |
| Location: | 4902187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453728 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGACAACACACCAGACAGCACACGGGTTACAACAGCACCAGCGCACCT |
| Internal bar code: | AAGAGGTCCTTATGTACTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 82.9545 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAGCAAAATTCGGCGG |
| Suggested primer 2: | TCAAAGGGAGGCGACTGTTC |