| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020710 |
| Chromosome: | chromosome 2 |
| Location: | 1011469 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079850 | POLK1 | (1 of 1) K03511 - DNA polymerase kappa (POLK); DNA polymerase kappa | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCGGCACGTCCCGCCCCGCAGAGTTTAGAGTTACCTAGCTATGTTA |
| Internal bar code: | AAGGGTCAGAAGTGCAATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 13.0952 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTGTGTGTTCTGCGCC |
| Suggested primer 2: | CGCCAGGCATCCAATTTAGC |