Insertion junction: CLIP2.020719_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020719
Chromosome:chromosome 6
Location:1065312
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g257250 BBS2 Bardet-Biedl syndrome-2 associated protein; (1 of 1) K16747 - Bardet-Biedl syndrome 2 protein (BBS2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCTCCCGGAACTCACTGGCCGCTCTTCCTCCCGAGCCCCTCCCCCAC
Internal bar code:GGGCGGGTCGAGTGTCTGCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:66
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTGTCAAGTCCAAACAC
Suggested primer 2:CATTCCACTACCCACCCACC