| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020741 |
| Chromosome: | chromosome 1 |
| Location: | 7850811 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055408 | ACS2 | (1 of 3) K01895 - acetyl-CoA synthetase (ACSS, acs); Acetyl-CoA synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGGTTCGTTTGCCGCAGTGGCACAGAGCGCAATGATGGTCACTGGGG |
| Internal bar code: | ATACCAGCAGCAGGTTTTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1860 |
| LEAP-Seq percent confirming: | 93.1818 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTTGTGCAGGACAAGC |
| Suggested primer 2: | CGTCGAAGTTGTAGCGCATG |