| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.020746 |
| Chromosome: | chromosome 1 |
| Location: | 2777742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017050 | MCP1 | (1 of 22) IPR018108//IPR023395 - Mitochondrial substrate/solute carrier // Mitochondrial carrier domain; Mitochondrial substrate carrier protein | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCACGGTTGCCCGCCCGGCGACGACTCCCCCGCCAAACAAAGCAACA |
| Internal bar code: | ATCCCAATTGTCTATAGCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1837 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGGGTGTGTTAGTGGG |
| Suggested primer 2: | CTCCGTCACACCCAATCACA |