Insertion junction: CLIP2.020749_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020749
Chromosome:chromosome 13
Location:78474
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801505 (1 of 2) PTHR10642//PTHR10642:SF7 - RIBONUCLEASE H1 // SUBFAMILY NOT NAMED CDS
Cre13.g801506 (1 of 2) PTHR10642 - RIBONUCLEASE H1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGAGGAGCCAGGGGCGGCGCCGCGCCGGGGCCGGGGGCGGCCGCGCAA
Internal bar code:CAGACACACGTGTACAGCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1449
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GACAACCTCTGACCCGCTAG
Suggested primer 2:CTGGAGGACGTGTTCTGCAT