Insertion junction: CLIP2.020780_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020780
Chromosome:chromosome 13
Location:2632015
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581050 GT90F46,GT90-46 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCCCAGTTCCCACACACGCGCCGAGAAGCCAGCGATTCCCGAATGTT
Internal bar code:CTTGGAGGGGTCCTAGATCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:292
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGCTCAATGAAGGGGTC
Suggested primer 2:TTTTGGTGGGTTGTCTGCCT