| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020781 |
| Chromosome: | chromosome 7 |
| Location: | 6431791 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357900 | (1 of 1) K08054 - calnexin (CANX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCCACACGCGCCCTCCCAGCTCCTGCCCGGTTTATTTTGGGATTTAA |
| Internal bar code: | AATTATTTATACTAATCAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2864 |
| LEAP-Seq percent confirming: | 93.0435 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACATGTGCAGGTGGGGTG |
| Suggested primer 2: | GGCAAGGGTAAGGCAAGGAT |