Insertion junction: CLIP2.020782_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020782
Chromosome:chromosome 10
Location:5372912
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g456950 CEP10 Cysteine endopeptidase; (1 of 1) IPR000668//IPR008979//IPR013128//IPR018392//IPR029062 - Peptidase C1A, papain C-terminal // Galactose-binding domain-like // Peptidase C1A // LysM domain // Class I glutamine amidotransferase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCCGCTTGAGCTGATGACCAACTACTACATCCCGCCCTGCACCAAGG
Internal bar code:TGAGTGGAGAGAGTGGTTACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4660
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCAAGACAAGCACACAC
Suggested primer 2:AAAGGTACGTACGTGCAGCA