Insertion junction: CLIP2.020788_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020788
Chromosome:chromosome 14
Location:715355
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g801604 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTTGAACGCTGAGGTCAAGATCTACGCCAGGTCCGAACGCGCCACATA
Internal bar code:GGTAGGTAGGTCTTGTAATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1851
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACCTGCGCCTCCCAG
Suggested primer 2:GTGGAGGTGGAGGAGGAGG