| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.020793 |
| Chromosome: | chromosome 7 |
| Location: | 1812984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325729 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAAGGAGGGAAGCAGCTGGGTGTTTGAGGCCCTGCAGTCGGGGACTG |
| Internal bar code: | ATGTTAATCGCAGATCATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1413 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTAGCCCTAGCAGCGCA |
| Suggested primer 2: | ACACTGAGGGCTTAGGTTGC |