Insertion junction: CLIP2.020796_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.020796
Chromosome:chromosome 2
Location:4567782
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107100 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCCCCACGGGGAGACATCTGACCGACTGTTCTCCTCCTCAAGCCGTAG
Internal bar code:CTTGGGCGGAGTTTAAGCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1850
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGAGGACTACAAGGAGGG
Suggested primer 2:TGTAGGCTGCATCAACCTGG