Insertion junction: CLIP2.020796_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020796
Chromosome:chromosome 5
Location:2421810
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g238650 PHC5 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 5 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCACCCACAGGTGGGAGCGAACACGGGTCAGCGTGGGAGGAGCGTC
Internal bar code:GCAGGACAGATAGGCATCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2588
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAAGATGGCAAACGAGC
Suggested primer 2:CCCCCAGACATGTACACACC