Insertion junction: CLIP2.020801_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020801
Chromosome:chromosome 1
Location:1662028
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g009200 POB29 Proteome of basal body 29; (1 of 2) IPR002083//IPR008974 - MATH/TRAF domain // TRAF-like 3'UTR/CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAACGGTGCTGTCCCGACCACCACCGGTGGCGCCTGCCACGACATTGC
Internal bar code:TTATTGAGATTGAGTATGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1616
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAATGAGCACTGACGCAT
Suggested primer 2:TAGATGCTTGGAGCGCAGAG