Insertion junction: CLIP2.020805_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020805
Chromosome:chromosome 10
Location:610436
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421700 GPDH1,GPD4 Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase; (1 of 4) 1.1.1.8 - Glycerol-3-phosphate dehydrogenase (NAD(+)) / NADH-dihydroxyacetone phosphate reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGCTGGAAGATCTTCTGGAACAGCGTGGCCGCATCCAGGTTGTCGTA
Internal bar code:GATAATATTGATCACTGCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:611
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TATGGTAGGGGCAGACGGAA
Suggested primer 2:CAAGCCAGAACGTGTGTGTG