Insertion junction: CLIP2.020821_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020821
Chromosome:chromosome 17
Location:241619
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697500 P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTGCCTCAGGTAAACAAACGAGAGGCAATACATGCTGGCATACTAGT
Internal bar code:TAATACCGTGTAGTTGAGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3397
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGTCCAGGACCCTACC
Suggested primer 2:GAAGTGTGTGAGTGCGAGGA