Insertion junction: CLIP2.020821_5

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020821
Chromosome:chromosome 17
Location:241624
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697500 P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTCGCGCGATCTTGCTCTCGCGCCTTCTCTACCGCTTCCTTCTCGTT
Internal bar code:GTAGTGCTATGGTAATAAAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2533
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTGTGTGAGTGCGAGGA
Suggested primer 2:GCTTTGTCCAGGACCCTACC