| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020842 |
| Chromosome: | chromosome 3 |
| Location: | 362173 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144687 | (1 of 2) PTHR12983:SF9 - RING FINGER PROTEIN 10 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCGGGGTGATGAAACTACGTATGGAGGGTTGTGATTTTGCTGTCGC |
| Internal bar code: | TAGAAATGGCGAGTCTCGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3403 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACAAAGTTCCAATGCA |
| Suggested primer 2: | CACCAAAATGGGATTCGCCG |