| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020846 |
| Chromosome: | chromosome 14 |
| Location: | 3450971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630301 | (1 of 1) IPR000104//IPR001890//IPR029058 - Antifreeze protein, type I // RNA-binding, CRM domain // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGTGTGGGGGTGTGCATGTGGGGTTTGGGGTTTGGGTTGTAGATA |
| Internal bar code: | GGTATTTGTACTTAATACGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTCCCATTCCCATGCT |
| Suggested primer 2: | GTGTGTTGTGGAACTTCCGC |