| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020852 |
| Chromosome: | chromosome 3 |
| Location: | 8367219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800397 | (1 of 1) K18735 - protein SMG9 (SMG9) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCATGCATAAGGCGGCTCCGGAGAGCCCTGTTGCGCAAGAGCAGATG |
| Internal bar code: | GCAATGGTCCGTGGAATACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 25.5814 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTGAGTGGTCCGTATGG |
| Suggested primer 2: | CAACAAAACGGGGAGCTGTG |