| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020853 |
| Chromosome: | chromosome 2 |
| Location: | 5742688 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116700 | PTK11 | (1 of 37) 2.7.11.17 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase; Protein tyrosine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACATACGGTACATCTTAGAGGTGAACAGACAGCTACCTGCCTGCATA |
| Internal bar code: | CGTAGGGTTATATCTTTGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4501 |
| LEAP-Seq percent confirming: | 96.648 |
| LEAP-Seq n confirming: | 173 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 179 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGTCATCCACCTACGC |
| Suggested primer 2: | ACTCGAACGTGGTTCAGACC |