Insertion junction: CLIP2.020857_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020857
Chromosome:chromosome 16
Location:1362665
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGACACGCCCACTCTGTACCACTCTACACTCAGTGCGCCATTCGTA
Internal bar code:AGATCTTTGGAAACGACGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2629
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAGTTGTGTGCCATACA
Suggested primer 2:AGGTGTGTGTGTGTGTGTGT