Insertion junction: CLIP2.020861_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020861
Chromosome:chromosome 6
Location:4221435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278245 PAO9,PAO5 Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGCTGACTGGCTGAGTCTGTGGCATGGGTGACTTTGCCTCATGCATA
Internal bar code:TAGAAATGGGCATTCCGCAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:184
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAACCCCCATTCCACTT
Suggested primer 2:CGACCTGCCCTACAGCTATG