Insertion junction: CLIP2.020869_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020869
Chromosome:chromosome 7
Location:5447140
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350867 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGCGGCACGCGGCGGCGTGAGGGACTGGGTGGAGGCGGTGGCGGCTG
Internal bar code:GCGGAGACGGCGTCCAACACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1797
LEAP-Seq percent confirming:86.3636
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AACACTACCGCCTGCAACAG
Suggested primer 2:GTCAACATGGGTGGAGGCAG