| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020873 |
| Chromosome: | chromosome 13 |
| Location: | 762865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566750 | (1 of 2) PTHR31385//PTHR31385:SF1 - FAMILY NOT NAMED // EXPRESSED PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGTGTTCTTGCGCTGACCGCTGCATCCCCCGACGAACCCAGCACACT |
| Internal bar code: | CAAGTGATTTGGTATGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1958 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCACCTGTTCCACGTTA |
| Suggested primer 2: | CACGCATGCTACGTCTTTCG |