Insertion junction: CLIP2.020878_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020878
Chromosome:chromosome 9
Location:6040844
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412600 CYG52 (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGTTTTGGGCTGCACCGGGTTCTCCTTGGGTCCGGCTCTTCGGCTGGT
Internal bar code:CATAAGTTAACGGATTGGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1138
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTTCTTCCGGCGTAGAC
Suggested primer 2:GACTTTGATGTGACGCTGCC