| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.020888 |
| Chromosome: | chromosome 3 |
| Location: | 2283284 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157650 | (1 of 30) IPR000253 - Forkhead-associated (FHA) domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTGCAGCGTGATCTGGGAAGGTTTGGGTGTTGGCCTGATTGCATTT |
| Internal bar code: | CACTTATCTGGCTAGGATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1111 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATAGGAGCTGGGGTTTT |
| Suggested primer 2: | CGTCTCCTGTGTTGCCTTCT |