Insertion junction: CLIP2.020895_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020895
Chromosome:chromosome 5
Location:462761
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g244000 (1 of 1) IPR000253//IPR009836 - Forkhead-associated (FHA) domain // Protein of unknown function DUF1399 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCGTGACTGCACACGCAGGGGTTCAGCCACCATCTGACTCACCAAACA
Internal bar code:GGTCTGCTCGTACTACTGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3508
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCCCAATTCTCTGTGCT
Suggested primer 2:TTGCTGTTTGTGGATGCGTG