| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020918 |
| Chromosome: | chromosome 3 |
| Location: | 1969567 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155527 | (1 of 1) IPR027363 - Methylthioribose-1-phosphate isomerase-like, N-terminal domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGTGGTGCTGAATCCTGACAACGTCTTGGGTGGCCCTGTCATCTGGC |
| Internal bar code: | GTTCAAAGGAGGGTTCGTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2453 |
| LEAP-Seq percent confirming: | 64.8649 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGATAATGACCCATCGT |
| Suggested primer 2: | GCACAGAGTGGACCCGTTAA |