| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.020922 |
| Chromosome: | chromosome 17 |
| Location: | 4400948 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730650 | (1 of 1) PTHR23250//PTHR23250:SF4 - DYSFERLIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACGCCGCGGCACTGCCTGTCAGTACCTCCGCCGCCATCGCCTCCGCC |
| Internal bar code: | ACGCTGGCGGGCATCAGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1715 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATCCACAGAGCCTCCG |
| Suggested primer 2: | ATCGGGTTACTTTGGGCTGG |