| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020927 |
| Chromosome: | chromosome 17 |
| Location: | 748882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701100 | CPK8 | (1 of 1) 2.7.1.83 - Pseudouridine kinase; putative carbohydrate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGAGCGGGCTTCGTGCTGGTTTAAAATGCGGTGTGCTGGTTGATGCC |
| Internal bar code: | AGACGAACTTGAGGTCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGTCAGTGGCGGTAAC |
| Suggested primer 2: | AGAGTGGGGTTTTAGGCGTG |