Insertion junction: CLIP2.020930_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.020930
Chromosome:chromosome 3
Location:4677838
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g177250 HAP3 (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase; Haloperoxidase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGGTAGCCGTTGTGCCAACAGCATGGGACCGTGCCGCGGCGCGCACA
Internal bar code:TAGGTCCATATTGTTATGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:179
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCATACACACGTCTGCG
Suggested primer 2:AGCCCAGCAGCTTGAAGTAG