| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020932 |
| Chromosome: | chromosome 12 |
| Location: | 2225244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508853 | BPL2 | Biotin-protein ligase; (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCGCCCTGCCTGCTGCGGGGATGTGGGGCCTGCAGGCAGCGGCATC |
| Internal bar code: | GAGCCCGTGTCCATTTCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1796 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTGGATGTACGGTGG |
| Suggested primer 2: | TGCGTGTGTGATGCTGAGAT |