Insertion junction: CLIP2.020942_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020942
Chromosome:chromosome 15
Location:5358149
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g734300 AGI1 (1 of 2) PTHR10903//PTHR10903:SF47 - GTPASE, IMAP FAMILY MEMBER-RELATED // TRANSLOCASE OF CHLOROPLAST 120, CHLOROPLASTIC-RELATED; Putative Avirulence-Induced Gene AGI1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCAACGGTAACGCAGGACCAACACACCAGGCAATGCGGAGGTGTCA
Internal bar code:ACGGGAATCGAGGCATTAAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1720
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGGCGTTCGACTTCATC
Suggested primer 2:CGTGTACTGATGCTGCATGC