Insertion junction: CLIP2.020951_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020951
Chromosome:chromosome 1
Location:2123855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g011850 (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACAAGCTCAGCGGCAAGGACGTCGAGTGTAGTTGTTGTCGAAATACT
Internal bar code:CCCTTAGGTTTTGCGTCGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:498
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGCGCGTATCCAACAGG
Suggested primer 2:ACGCTACTGAGAATACGGCG