| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.020981 |
| Chromosome: | chromosome 13 |
| Location: | 570031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACGCAAGCGCACAAGAACGAAGCTGGGGCCTGTGCCCATGCATGCT |
| Internal bar code: | GAGAAGCCTAATTTGCTCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1520 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGGAAGAAAGCGCTGC |
| Suggested primer 2: | TTTTCCTCCTCGAACCTGGC |