| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021026 |
| Chromosome: | chromosome 17 |
| Location: | 4161700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802079 | (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGCTGCGTAGGTCCACGTGATGCCTAACTGCGTGCCCGCAGACGCG |
| Internal bar code: | TCGAGGCACACGTTTTTAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4247 |
| LEAP-Seq percent confirming: | 98.9362 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTTACATGTGCATGCGG |
| Suggested primer 2: | ATGTCTCCCAAGCCCAAGTG |