Insertion junction: CLIP2.021032_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.021032
Chromosome:chromosome 10
Location:1233095
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g426400 TRL8,TRX22 (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCCCAAACCCAGCCACCTACACACGCACACGCGCACACACGCCCATCC
Internal bar code:CTAGTATGTATCGGGTTGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2335
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CTTAACATCGCGCTGACAGC
Suggested primer 2:CGTATGTTGCAGCGACACAG