| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.021034 |
| Chromosome: | chromosome 2 |
| Location: | 3655151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100000 | RFA2,RPA30 | Replication protein A, 30 kDa subunit; (1 of 1) K10739 - replication factor A2 (RFA2, RPA2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTATCTACGTGCCTCGCCAAAACCTTCTACTCCTCTAGCTTTGCGT |
| Internal bar code: | GTAAGAAGGCATAAACGTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1712 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCTCCGGGGGTATCAT |
| Suggested primer 2: | TGCAGGAAGTGGTACGTCAC |