Insertion junction: CLIP2.021039_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021039
Chromosome:chromosome 11
Location:1214991
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGTGAAGCTCTCCTTCCCCTTGAGCTTGGGAACCGCCTTGGTCTGTG
Internal bar code:TATTCCGGGATGTTGTTCCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:171
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCAGGCACTCCTACAATT
Suggested primer 2:CGCGCAACCTAACACACAAA