Insertion junction: CLIP2.021046_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021046
Chromosome:chromosome 7
Location:1804230
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325728 (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAACCACCGTGTGCCCCTGAACCATCCCCCACCCGCTCCCGAATCACA
Internal bar code:AATACCCCCCTTGCTGTTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2010
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCGTCACCAATCCCAC
Suggested primer 2:CCGTGAAGACCAGGAGCTTT