| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021051 |
| Chromosome: | chromosome 6 |
| Location: | 3010733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274450 | SMM20 | (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGCGCCCAGCTACGGCTGGCGGTGGGCGTGCGGGACCTAGGCGGGG |
| Internal bar code: | AAATTGACTACGCTAGACGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1842 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACCTCGAAGCAATCCT |
| Suggested primer 2: | ACGCGTCTGGGACTAGTACT |