Insertion junction: CLIP2.021066_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021066
Chromosome:chromosome 9
Location:3629156
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396957 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTTATGTGTCCTCCCGGTCCTGACTGCTACGCAAAGCGAACTGGTGA
Internal bar code:TGGTTAAACGATGTGCGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:962
LEAP-Seq percent confirming:31.5789
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGCCGTCGTACATGTGA
Suggested primer 2:TGCCATGCCACATACACACT