Insertion junction: CLIP2.021079_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.021079
Chromosome:chromosome 6
Location:6646107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g294850 UBC10 Ubiquitin-conjugating enzyme E2; (1 of 1) PTHR24067:SF142 - UBIQUITIN-CONJUGATING ENZYME E2 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCCCAACGGTGAGCACGCGCCTCCCCCAACGCCCCACAGCTTCCCAC
Internal bar code:CGAGGTTCCGATTGTGGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2472
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGCTTTCAACACATGCA
Suggested primer 2:CTGAAATCCGCCAGCGATTG