| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021095 |
| Chromosome: | chromosome 9 |
| Location: | 2885361 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801026 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAACCCAGCCACAAACACTGTACTGTACACACGCAATACACACAAACA |
| Internal bar code: | ATGCGATTCATCCTGGTTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACCATGAGCTTCGCC |
| Suggested primer 2: | GTGTGTGTGCGTGTGTGTAC |