Insertion junction: CLIP2.021110_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021110
Chromosome:chromosome 17
Location:2775962
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g718000 PHC7,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGTAGGGCGCTGCACACGTCCCGTGAACATCAGATGCAAGCCTCTGC
Internal bar code:TAGGGGTGGAGTAGGATACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:564
LEAP-Seq percent confirming:24.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGGTCACGGACAACACG
Suggested primer 2:CCCTTGCGAGGTAGGTCAAG