Insertion junction: CLIP2.021112_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021112
Chromosome:chromosome 1
Location:2326892
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g013000 (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCATTGCCCTGCGCCCACTCCAACCGCTCCACCAGTCCTTCCACCGC
Internal bar code:CGGCAGCCCTGGGACACCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTTGGATGCCAAAAGCG
Suggested primer 2:GCACAGCGACACACAAACAT