| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021116 |
| Chromosome: | chromosome 12 |
| Location: | 1297397 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489450 | (1 of 2) IPR017993 - Atrophin-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCCGGAGCAGCGGTCGGCGCGCGAGTCCGTCAGGGCGGATGCGGCG |
| Internal bar code: | TGGGCCGTGCAGTACACTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4216 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCGCCATCCTCATCCTC |
| Suggested primer 2: | GTCTATACGCGGGCATGTCA |