Insertion junction: CLIP2.021117_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.021117
Chromosome:chromosome 12
Location:7889378
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553450 CNG1 Cyclic-nucleotide gated potassium channel; (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCACCGCCAACCAGCGGCGCTCCAGCGCGCTTTCGAGCGGCGCCCAA
Internal bar code:TACAAGGGTACGTGTGTCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:790
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCTGGAGTGCATATGAA
Suggested primer 2:GTCTCAGCGGTGTTTCCAGA