Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | + |
Strain: | CLIP2.021152 |
Chromosome: | chromosome 9 |
Location: | 62633 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g386161 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACTGGTGGGCCGCGCCGAGGGCAGCATAACGTACCACCGGCGGAAGG |
Internal bar code: | CGATCGTGGCTTCATTGCCCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 384 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACACTTGTCATCTCCGCGT |
Suggested primer 2: | GTTTGGGAACGTGACAAGGC |